MTA can modulate tissue repair and hard tissue deposition due to its ability to promote the precipitation of carbonate apatite through the release of calcium ions and their interaction with phosphate in the dentin matrix or tissue ( 3). The first formulation of this type of material was patented in 1995 and was the grey MTA, which consisted of Portland cement and bismuth oxide ( 1, 2). These types of cement are radio-opaque and form a calcium silicate gel when mixed with water. Tricalcium silicate-based materials such as mineral trioxide aggregate (MTA) have biological, physicochemical, and mechanical properties that are well suited to clinical applications. Palabras clave Apatita carbonatada Bioactividad Biodentine Biomineralización MTA Flow Push-out Se concluye que el MTA Flow en consistencia Putty o Thick presenta un proceso de biomineralización y una resistencia al desplazamiento similar al MTA Angelus y al Biodentine, sin embargo, esta última disminuye considerablemente en presentación Thin. En general se observaron zonas de biomineralización en la interface, sin embargo, no fueron continuas. De acuerdo a las pruebas de resistencia al desplazamiento, a las 72 horas post-obturación, solamente se encontró diferencia significativa entre las muestras obturadas con Biodentine y las correspondientes al MTA Flow consistencia Thin (p=0.0090), sin embargo las muestras sumergidas 15 días en PBS presentaron diferencia significativa entre todos los grupos con respecto al MTA Flow Thin (p=0.0147). Las muestras con Biodentine presentaron la mayor liberación de iones calcio. No obstante, si presentó una diferencia significativa con respecto al grupo de MTA Flow® consistencia Thin (P<0.05). Las muestras obturadas con Biodentine promovieron una mayor precipitación de apatita carbonatada, sin embargo, no se presentó diferencia estadística significativa con respecto al MTA Angelus, MTA Flow® Putty ni Thick (p=0.0536). Las muestras se sometieron al proceso de biomineralización y a pruebas de Push-out. Se utilizaron discos de dentina de 2mm de espesor del tercio medio radicular con cavidades estandarizadas de 1.5mm de diámetro, las cuales se obturaron con diferentes materiales entre ellos: MTA Angelus (Angelus Dental, Brasil), Biodentine (Septodont, Francia), MTA Flow® Consistencia Putty (Ultradent, E.E.U.U), MTA Flow® Consistencia Thick (Ultradent, E.E.U.U) y MTA Flow® Consistencia Thin (Ultradent, E.E.U.U). Keywords Bioactivity Biodentine Biomineralization Carbonate apatite MTA Flow Push-out testĮl objetivo de este estudio fue evaluar, en un modelo ex vivo, el proceso de biomineralización y fuerza de adhesión del MTA Flow® en sus tres diferentes consistencias por medio de la prueba de resistencia al desplazamiento (Push-out). MTA Flow® Thin, despite their tubular infiltration, results in gaps and a defective peripheral seal.Therefore, MTA Flow®, in Putty or Thick consistencies, presents a biomineralization process and push-out strength similar to MTA Angelus and Biodentine, however, both characteristics decreases considerably in Thin consistency. However, the thickness of the interface was lower compared to that of Biodentine. MTA Flow® Putty and Thick consistencies show a good adaptation to dentin, similar to MTA-Angelus. Putty or Thick consistencies presented a similar bonding strength to MTA-Angelus and Biodentine. The samples submerged in phosphate-buffered saline (PBS) for 15 days showed a significant difference between all groups and MTA Flow® Thin (p=0.0147). After 72 hours, a significant difference was found between the Biodentine and MTA Flow® Thin groups (p=0.0090) in the push-out test. Zones of biomineralization were observed at the interface but were not continuous. The formation of a partially carbonated intermediate apatite layer was observed in all groups. The samples with Biodentine displayed the greatest release of calcium ions. However, there was no significant difference between MTA-Angelus, MTA Flow® Putty, or Thick (p=0.0536), but there was a significant difference in the Thin group (P<0.05). The samples filled with Biodentine had a higher precipitation of carbonate apatite. The samples were divided into 3 groups: subgroup 1 (n=5), analysis of the biomineralization process 2 (n=20), evaluation of the bonding strength and push-out resistance and 3 (n=5), evaluation of the cement/ dentin interface. Samples were filled with MTA-Angelus (Angelus Dental, Brazil), Biodentine (Septodont, France), MTA Flow® Putty (Ultradent, USA), MTA Flow® Thick or MTA Flow® Thin. The push-out test was performed on an ex vivo model, using 2mm thick dentin discs from the middle third of the root with standardized cavities of 1.5 mm. This study evaluated the biomineralization processes and push-out strength of MTA Flow® with radicular dentine in three different consistencies.
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